Electronic Automated Measurement User System (EAMUS™)

COMET FISH

The COMET FISH program is an appropriate program to measure the DNA and FISH signals in images obtained from the single cell
gel electrophoresis (Comet assay). It requires high magnified images (objective *40, or higher) and can be applied with or without
syntactic structure analysis. It provides the measurement data of a single or of two probes (red, green labelled) and measures the
DNA distribution in the nucleus and tail as well as FISH signals located within the nucleus (DAPI stained) and outside the nucleus
if present. It can be applied
with or without syntactic structure analysis.

The measurement data of the syntactic structure analysis are described separately.

The measurement program will provide you with the following results:

Measurement data

mean size nucleus Vv: mean Volume fraction of nuclear area
percent DNA in nucleus: percent of DNA in nucleus (in relation to DNA (nucleus) + DNA (tail))
mean IOD in nucleus: mean integrated optical density (IOD) of DNA in the nucleus
neg. nuclear DNA area: nuclear area without DNA in the nucleus
range nuclear DNA: range of integrated optical density (IOD) in the nucleus
mean size tail Vv: Volume fraction of tail area
percent DNA in tail: percent of DNA in tail (in relation to DNA (nucleus) + DNA (tail))
mean IOD in tail: mean integrated optical density (IOD) of DNA in the tail
neg. tail DNA area: tail area without DNA
range tail DNA: range of integrated optical density (IOD) in the tail
No DNA agglutinations tail: number of DNA agglutinations in the tail
rel. distance tail center<> nuclear center: relative distance of center of gravity in the tail to the center of gravity in
the nucleuas (in relation to the nuclear diameter)
cor. coefficient dx<>area tail DNA: correlation coefficient between area of DNA agglutinations in the tail and their
distance to the nuclear center
cor. coefficient dx<>IOD tail DNA: correlation coefficient between IOD of DNA agglutinations in the tail and their distance
to the nuclear center
cor. coefficient dx<>neg DNA area tail: correlation coefficient between negative area of DNA agglutinations in the tail and
their distance to the nuclear center
cor. coefficient dx<>range tail DNA: correlation coefficient between IOD range of DNA agglutinations in the tail and their
distance to the nuclear center
n-star tail DNA agglutinations: mean of No of neighbouring DNA agglutinations in the tail
rel. edge length tail DNA: mean of relative distance between DNA agglutinations in the tail
entropy IOD DNA agglutinations: mean structural entropy of DNA agglutinations in the tail
No nuclear FISH-1-: absolute No of FISH signals of the first probe (if two probes ->red, if only one probe -> red or green)
in the nucleus
rel. distance FISH-1-<>nuclear center: mean of relative distance of nuclear FISH signals No 1 to the nuclear center
No FISH-1- tail: absolute No of FISH signals of the first probe (if two probes ->red, if only one probe -> red or green) in the tail
rel. distance FISH-1-tail<>nuclear center: mean of relative distance of FISH signals No 1 in the tail to the nuclear center
area FISH-1-in nucleus: mean of absolute FISH area (probe 1) in the nucleus
IOD FISH-1- in nucleus: mean of IOD of FISH signals (probe 1) in the nucleus
area FISH-1- in tail: mean of area of FISH signals (probe 1) in the tail
IOD FISH-1- in tail: mean of IOD of FISH signals (probe 1) in the tail
No nuclear FISH-2-: absolute No of FISH signals (probe 2) in the nucleus
rel. distance nuclear FISH-2-<>nuclear center: mean of relative distance of nuclear FISH signals (probe 2) to the nuclear center
No FISH-2- tail: absolute No of FISH signals (probe 2) in the tail
rel. distance FISH-2-<>nuclear center: mean of relative distance of nuclear FISH signals (probe 2) in the tail to the nuclear center
area FISH-2-in nucleus: mean of absolute FISH area (probe 2) in the nucleus
IOD FISH-2- in nucleus: mean of IOD of FISH signals (probe 2) in the nucleus
area FISH-2- in tail: mean of area of FISH signals (probe 2) in the tail
IOD FISH-2- in tail: mean of IOD of FISH signals (probe 2) in the tail