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Impressum 

Electronic Automated Measurement User System (EAMUS™)
FISH
The FISH program is an appropriate program to measure the fluorescence in situ hybridization of nuclei in relation to the spatial
distribution within the nuclei. It requires high magnified images (objective *40, or higher) and can be applied with or without syntactic
structure analysis. It provides the measurement data of a single or of two probes (red, green labelled) and measures only those events
located within a nucleus (DAPI stained) and outside the nucleus if present. It can be applied with or without syntactic structure analysis.
The measurement data of the syntactic structure analysis are described separately.
The measurement program will provide you with the following results:
Measurement data
 mean nuclear area: mean area of nuclei (absolute size)
 stnd nuclear area: standard deviation of mean nuclear area
 mean nuclear radius: mean nuclear radius (absolute length)
 stnd nuclear radius: standard deviation of mean nuclear radius
 Vv nuclei: Volume fraction of nuclei (DAPI)
 mean No nuclei: mean number of nuclei per image
 mean size FISH red: mean area of FISHred signal (absolute size)
 stnd size FISH red: standard deviation of area of FISHred signal
 Vv FISH red/nucleus: Volume fraction of FISHred signals per nucleus
 total No FISH red: total number of red FISH signals per image
 mean No FISH red/nucleus: mean number of FISHred signals per nucleus;
 percent FISH red outside nuclei: percent of red FISH signals outside the nucleus
 percent nuclei without FISH: percent of nuclei without red FISH signals
 percent nuclei (1* FISH red): percent of nuclei with one FISHred signal
 percent nuclei (2* FISH red): percent of nuclei with two FISHred signals
 percent nuclei (3* FISH red): percent of nuclei with three FISHred signals
 percent nuclei (4* FISH red): percent of nuclei with four FISHred signals
 percent nuclei (5* FISH red): percent of nuclei with five FISHred signals
 percent nuclei (6* FISH red): percent of nuclei with six FISHred signals
 percent nuclei (7* FISH red): percent of nuclei with seven FISHred signals
 percent nuclei (8* FISH red): percent of nuclei with eight FISHred signals
 percent nuclei (9* FISH red): percent of nuclei with nine FISHred signals
 percent nuclei (10* FISH red): percent of nuclei with ten FISHred signals
 mean size FISH green: mean area of FISHgreen signal;
 stnd size FISH green: standard deviation of area of FISHgreen signal
 Vv FISH green/nucleus: Volume fraction of FISHgreen signals per nucleus
 total No FISH green: total number of green FISH signals per image
 mean No FISH green/nucleus: mean number of FISHgreen signals per nucleus
 percent FISH green outside nuclei: percent of green FISH signals outside the nucleus
 percent nuclei without FISH: percent of nuclei without green FISH signals
 percent nuclei (1* FISH green): percent of nuclei with one FISHgreen signal
 percent nuclei (2* FISH green): percent of nuclei with two FISHgreen signals
 percent nuclei (3* FISH green): percent of nuclei with three FISHgreen signals
 percent nuclei (4* FISH green): percent of nuclei with four FISHgreen signals
 percent nuclei (5* FISH green): percent of nuclei with five FISHgreen signals
 percent nuclei (6* FISH green): percent of nuclei with six FISHgreen signals
 percent nuclei (7* FISH green): percent of nuclei with seven FISHgreen signals
 percent nuclei (8* FISH green): percent of nuclei with eight FISHgreen signals
 percent nuclei (9* FISH green): percent of nuclei with nine FISHgreen signals
 percent nuclei (10* FISH green): percent of nuclei with ten FISHgreen signals

