Electronic Automated Measurement User System (EAMUS™)

Nuclei

The nuclear staining program is an appropriate program to measure antigens that are present in the nuclei. It requires moderately
to high magnified images (objective *20, *40, or higher) and can be applied with or without syntactic structure analysis. Appropriate
markers are those for proliferating (or apoptotic) cells, or sexual hormones, (Ki-67, bcl_2, oestrogen, progesterone receptors, etc.).

The measurement program will provide you with the following results:

The measurement data of syntactic structure analysis are described separately.

Measurement data

No nuclei: Number of measured nuclei
No positive nuclei: Number of measured nuclei with positive staining
Vv nuclei neg: Volume fraction of negative nuclei 100 *[S{(pixnu)/(pixtot)}]
Vv positive stain: Volume fraction of all stained pixels (independent from nuclear, membrane, etc. stain)*100
% Vv pos nuclei: percent of Vv of positive stained nuclei (Vv of positive stained nuclei/Vv all nuclei)*100
% weak stain: Percent of weakly stained nuclei
% mod. stain: percent of moderately stained nuclei
% inten stain: percent of intensively stained nuclei
mean nuclear distance: mean distance between gravity centers of nuclei
mean nuclear area: mean area of all detected nuclei (either in pixels or in absolute square microns)
stnd dev nuclear area: standard deviation of mean nuclear area
mean max diameter: mean of maximum nuclear diameter
stnd dev max diameter: standard deviation of mean of maximum nuclear diameter
mean min diameter: mean of minimum nuclear diameter
stnd dev min diameter: standard deviation of mean of minimum nuclear diameter
percent pos nuclei: percent of positively stained nuclei (>10% of nuclear area)
percent mean pos nucl area: {mean of positive-stained nuclear area/mean of total area of positive stained nuclei}*100
mean pos nucl area: mean of area of positive stained nuclei
stnd pos nucl area: standard deviation of mean of area of positive stained nuclei
mean neg nucl area: mean of area of negative stained nuclei
stnd neg nucl area: standard deviation of mean of area of negative stained nuclei
p diff nucl area: p-value (t-test) on difference between areas of positive – negative stained nuclei;
mean pos min diameter: mean of minimum diameter of positive stained nuclei
mean pos max diameter: mean of maximum diameter of positive stained nuclei
stnd pos max diameter: standard deviation of mean of maximum diameter of positive stained nuclei
mean neg max diameter: mean of maximum diameter of negative stained nuclei;
stnd neg max diameter: standard deviation of mean of maximum diameter of negative stained nuclei
p diff max diameter: p-value (t-test) on difference between maximum diameters of positive – negative stained nuclei
mean pos min diameter: mean of minimum diameter of positive stained nuclei
stnd neg max diameter: standard deviation of mean of minimum diameter of positive stained nuclei
mean neg min diameter: mean of minimum diameter of negative stained nuclei
stnd neg max diameter: standard deviation of mean of minimum diameter of negative stained nuclei
p diff min diameter: p-value (t-test) on difference between minimum diameters of positive – negative stained nuclei
grading (%Vv * %int nuclei): quantitative grading for immunohistochemistry reaction
(percent of positive stained nuclei Np) * (positive area fraction within the nuclear area of positive stained nuclei).