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Impressum 

Electronic Automated Measurement User System (EAMUS™)
Nuclei
The nuclear staining program is an appropriate program to measure antigens that are present in the nuclei. It requires moderately
to high magnified images (objective *20, *40, or higher) and can be applied with or without syntactic structure analysis. Appropriate
markers are those for proliferating (or apoptotic) cells, or sexual hormones, (Ki67, bcl_2, oestrogen, progesterone receptors, etc.).
The measurement program will provide you with the following results:
The measurement data of syntactic structure analysis are described separately.
Measurement data
 No nuclei: Number of measured nuclei
 No positive nuclei: Number of measured nuclei with positive staining
 Vv nuclei neg: Volume fraction of negative nuclei 100 *[S{(pixnu)/(pixtot)}]
 Vv positive stain: Volume fraction of all stained pixels (independent from nuclear, membrane, etc. stain)*100
 % Vv pos nuclei: percent of Vv of positive stained nuclei (Vv of positive stained nuclei/Vv all nuclei)*100
 % weak stain: Percent of weakly stained nuclei
 % mod. stain: percent of moderately stained nuclei
 % inten stain: percent of intensively stained nuclei
 mean nuclear distance: mean distance between gravity centers of nuclei
 mean nuclear area: mean area of all detected nuclei (either in pixels or in absolute square microns)
 stnd dev nuclear area: standard deviation of mean nuclear area
 mean max diameter: mean of maximum nuclear diameter
 stnd dev max diameter: standard deviation of mean of maximum nuclear diameter
 mean min diameter: mean of minimum nuclear diameter
 stnd dev min diameter: standard deviation of mean of minimum nuclear diameter
 percent pos nuclei: percent of positively stained nuclei (>10% of nuclear area)
 percent mean pos nucl area: {mean of positivestained nuclear area/mean of total area of positive stained nuclei}*100
 mean pos nucl area: mean of area of positive stained nuclei
 stnd pos nucl area: standard deviation of mean of area of positive stained nuclei
 mean neg nucl area: mean of area of negative stained nuclei
 stnd neg nucl area: standard deviation of mean of area of negative stained nuclei
 p diff nucl area: pvalue (ttest) on difference between areas of positive – negative stained nuclei;
 mean pos min diameter: mean of minimum diameter of positive stained nuclei
 mean pos max diameter: mean of maximum diameter of positive stained nuclei
 stnd pos max diameter: standard deviation of mean of maximum diameter of positive stained nuclei
 mean neg max diameter: mean of maximum diameter of negative stained nuclei;
 stnd neg max diameter: standard deviation of mean of maximum diameter of negative stained nuclei
 p diff max diameter: pvalue (ttest) on difference between maximum diameters of positive – negative stained nuclei
 mean pos min diameter: mean of minimum diameter of positive stained nuclei
 stnd neg max diameter: standard deviation of mean of minimum diameter of positive stained nuclei
 mean neg min diameter: mean of minimum diameter of negative stained nuclei
 stnd neg max diameter: standard deviation of mean of minimum diameter of negative stained nuclei
 p diff min diameter: pvalue (ttest) on difference between minimum diameters of positive – negative stained nuclei
 grading (%Vv * %int nuclei): quantitative grading for immunohistochemistry reaction
(percent of positive stained nuclei Np) * (positive area fraction within the nuclear area of positive stained nuclei).

