Electronic Automated Measurement User System (EAMUS™)

Vessels

The vascular staining program is an appropriate program to measure the vascularization of lesions in relation to the spatial
distance of nuclei from their nearest neighbouring (supporting) vessel. It requires low to moderately magnified images
(objective *10, *20,) and can be applied with or without syntactic structure analysis. Appropriate marker are those for endothelial
cells (CD31, CD34, Factor IIX), and, if wanted additional nuclear stain (Ki-67, etc.). Images showing the diffusion gradients around
the vessels can be included into the output data.

The measurement data of syntactic structure analysis are described separately.

The measurement program will provide you with the following results:

Measurement data

Vv vessel: Vascular volume fraction (density) {100*vascular area / image area}
Sv (La) vessel: Vascular surface to volume fraction {1000 * (total of vascular surface)} / (total image volume), or
1000 * (total vessel circumference) / (image area)
mean vascular area: absolute measure of mean of vascular area
stnd dev vascular area: standard deviation of mean of vascular area
mean vascular surface: absolute measure of mean of vascular circumference
stnd dev vascular surface: standard deviation of mean of vascular circumference
mean min vasc diameter: absolute measure of mean of minimum vascular diameter
stnd dev min vasc diameter: standard deviation of mean of minimum vascular diameter
mean vessel distance: absolute measure of mean of distance between neighbouring vessels
stnd dev vessel distance: standard deviation of mean of distance between neighbouring vessels
Vv nuclei: Nuclear volume fraction
mean nucl area: absolute measure of mean of nuclear area
stnd dev nucl area: standard deviation of mean of nuclear area
mean nucl holes: absolute measure of mean of nuclear area with grey values below the (surrounding) threshold
stnd dev nucl holes: standard deviation of mean of nuclear area with grey values below the (surrounding) threshold
mean nucl homogeneity: absolute measure of mean of maximum difference between highest – lowest grey value
within the nuclear area
stnd dev nucl homogeneity: standard deviation of mean of maximum difference between highest – lowest grey value
within the nuclear area
mean nucl stain: absolute measure of mean of nuclear grey values (above the threshold (surrounding area))
stnd dev nucl stain: standard deviation of mean of nuclear grey values (above the threshold (surrounding area))
mean nuclear diff gradient: absolute measure of mean of nuclear distance to nearest neighbouring vessel (
0= no distance; 255=largest distance)
stnd dev nucl diff gradient: standard deviation of mean of nuclear distance to nearest neighbouring vessel
(0= no distance; 255=largest distance)
corr coef nucl density - vascular distance: linear correlation coefficient between nuclear density and nuclear
distance to nearest neighbouring vessel
y-regression a*x: constant “a” of linear regression formula between nuclear density and nuclear distance to nearest
neighbouring vessel, i.e., y=a*x+b
constant b: constant “b“ of linear regression formula between nuclear density and nuclear distance to nearest
neighbouring vessel, i.e., y=a*x+b.